General description
c-Myc (bHLHe39) is a transcription factor that regulates the expression of multiple genes involved in apoptosis, cell growth, differentiation, and proliferation. Active c-Myc is coupled to the MAX protein via c-Myc’s C-terminal helix-loop-helix leucine zipper (bHLHLZ) domain. The c-Myc-MAX heterodimer also interacts with a number of other transcription-related proteins including TRRAP, p107 and Miz-1, and c-Myc-MAX may indirectly regulate histone acetylases involved in chromatin remodeling. c-Myc is itself acetylated on lysine 323 by GCN5, and on lysine 148 and 157 by p300/CBP. The significance of c-Myc acetylation is still under investigation; however, previous studies have suggested that HAT-dependent acetylation of c-Myc may regulate its turnover and stability. c-Myc may play a role in various cancers such as Burkitt’s lymphoma.
~58 kDa observed. Uniprot describes two isoforms at 49 and 51 kDa
Immunogen
Epitope: Acetylated Lys323
KLH-conjugated linear peptide corresponding to human c-Myc acetylated at Lys323.
Application
Peptide Inhibition Assay Analysis: 2 µg/mL from a representative lot blocked acetyl-c-Myc (Lys323) in HEK293T co-transfected with wild type c-Myc or mutant c-Myc (Lys323R) and P300.
Peptide Inhibition Assay Analysis: A representative lot was used by an independent laboratory in HEK293T cells co-transfected with mouse wildtype c-Myc and P300. (Image courtesy of Dr. Ernest Martinez, Department of Biochemistry, University of California at Riverside.)
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Use Anti-acetyl-c-Myc (Lys323) Antibody (Rabbit Polyclonal Antibody) validated in WB to detect acetyl-c-Myc (Lys323) also known as HLHe39, Proto-oncogene c-Myc, Transcription factor p64, c-MYC.
Biochem/physiol Actions
Other homologies: Rat, Feline, Sheep, and Bovine (90% sequence homology).
This antibody recognizes c-Myc acetylated at Lys323.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blot in transfected HEK293T cell lysates.
Western Blot Analysis: 0.2 µg/mL of this antibody detected c-Myc on 10 µg of transfected HEK293T cell lysates.
Control
Transfected HEK293T cell lysates
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 100 |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | mouse, human |
| species reactivity (predicted by homology) | rhesus macaque (based on 100% sequence homology), chimpanzee (based on 100% sequence homology) |
| technique(s) | western blot: suitable |
| NCBI accession no. | NP_002458 |
| UniProt accession no. | P01106 |
| shipped in | wet ice |
| target post-translational modification | acetylation (Lys323) |
| Gene Information | chimpanzee ... Myc(464393) human ... MYC(4609) mouse ... Myc(17869) rhesus monkey ... Myc(694626) |
