General description
Caspase-8 (MACH/FLICE/Mch5) is a member of a family of cysteine-requiring aspartate proteases that participate in the intracellular signaling cascade leading to apoptosis, or programmed cell death. Caspase-8 is recruited to death-inducing signaling complex (DISC), CD95 (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) and is subsequently activated by proteolytic cleavage into two residues, p10 and p18. Active caspase-8 or the p18 subunit, is the first in the caspase signaling cascade, and it signals downstream caspases to eventually induce apoptosis.
~18 kDa observed
Immunogen
KLH-conjugated linear peptide corresponding to the active form of human Caspase-8.
Application
Detect Caspase-8 (active form p18 subunit) using this Anti-Caspase-8 (active form p18 subunit) Antibody, clone 2B12.1 validated for use in WB & IC.
Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected active Caspase-8 (p18 subunit) in etoposide-treated HeLa cells.
Research Category
Apoptosis & Cancer
Research Sub Category
Caspases
Apoptosis - Additional
Biochem/physiol Actions
This antibody recognizes the active form (p18 subunit) of Caspase-8.
Physical form
Format: Purified
Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Camptothecin-treated and untreated Jurkat lysates
Evaluated by Western Blot in camptothecin-treated and untreated Jurkat lysates.
Western Blot Analysis: 2 µg/mL of this antibody detected the active form of Caspase-8 (p18 subunit) in 10 µg of camptothecin-treated Jurkat lysates. Untreated jurkat lysate exhibited no detection of active Caspase-8.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 04-573; 04-574
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 2B12.1, monoclonal |
| species reactivity | human |
| technique(s) | immunocytochemistry: suitable,western blot: suitable |
| isotype | IgMκ |
| NCBI accession no. | NP_001073593 |
| UniProt accession no. | Q14790 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... CASP8(841) |
