Immunogen
Synthetic peptide from human filamin B. The immunogen peptide corresponds to a sequence near the hinge 2 region of Filamin B (near C-term) and is not present in the two C-terminally truncated splice variants of Filamin B (Var-2 and Var-3). The peptide shows no significant similarity to other antigens in the human protein database including Filamin A or Filamin C.
Application
Anti-Filamin B Antibody detects level of Filamin B & has been published & validated for use in WB.
Research Category
Cell Structure
Research Sub Category
Cytoskeleton
Western blot: 0.1 μg/mL on HeLa cell lysate and LAN-1 human neuroblastoma cell lysate. 0.25-0.5 μg/mL on HuVec cell lysate. Reacts with the ~278 kDa Filamin B protein.
Optimal working dilutions must be determined by the end user.
Biochem/physiol Actions
Filamin B
Physical form
Affinity purified immunoglobulin. Liquid in 0.02 M PBS, 0.25 M NaCl containing 0.1% sodium azide.
Preparation Note
Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.
Analysis Note
Control
HeLa cells
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human |
| manufacturer/tradename | Chemicon® |
| technique(s) | western blot: suitable |
| NCBI accession no. | NM_001457.2 |
| UniProt accession no. | O75369 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... FLNB(2317) |
