产品介绍
Product Description
General description
Recombinant, β1-3,6-Galactosidase expressed in E. coli. Catalyzes the hydrolysis of non-reducing terminal β1,3- and β1,6-linked galactose from complex carbohydrates and glycoproteins. Not known to cleave substituted or branched galactose. Inhibited by fucose attached to penultimate sugar.
Physical form
In 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.
Other Notes
One unit is defined as the amount of enzyme that will catalyze the release of 1.0 µmol p-nitrophenol from p-nitrophenyl-β-D-galactopyranoside per min at 37°C, pH 5.0.
Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
技术参数
Specifications
| recombinant | expressed in E. coli |
| Quality Segment | 100 |
| form | liquid |
| specific activity | ≥30 units/mL,≥75 units/mg protein |
| manufacturer/tradename | Calbiochem® |
| storage condition | do not freeze |
| foreign activity | Proteases, none detected |
| shipped in | wet ice |
| storage temp. | 2-8°C |
