Immunogen
Epitope: isoform NHE3
Maltose Binding Protein fusion protein containing C-terminal 131 amino acids of rabbit NHE3.
Application
Anti-Na+/H+ Exchanger-3 Antibody, isoform NHE3, clone 4F5 detects level of Na+/H+ Exchanger-3 & has been published & validated for use in IC, IH(P), IP & WB.
Immunoblotting (rabbit 80 kDa and rat ~83 kDa)
Immunohistochemistry (rat) on PLP or paraformaldehyde fixed tissue. Works on paraffin embedded tissue sections.
Immunocytochemistry
Immunoprecipitation (use non-ionic detergent)
Optimal working dilutions must be determined by the user.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters
Biochem/physiol Actions
Na+/H+ exchanger, isoform NHE3.
Physical form
Format: Purified
Liquid in 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide.
Preparation Note
Maintain at 2-8°C in undiluted aliquots for up to 6 months.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: MAB3134
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 4F5, monoclonal |
| species reactivity | rat, rabbit |
| manufacturer/tradename | Chemicon® |
| technique(s) | immunocytochemistry: suitable,immunohistochemistry: suitable (paraffin),immunoprecipitation (IP): suitable,western blot: suitable |
| isotype | IgG1 |
| NCBI accession no. | NM_001082697.1 |
| UniProt accession no. | P26432 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | rabbit ... Slc9A3(100009430) rat ... Slc9A3(24784) |
