General description
Amyloid β 1-40 is one of several functional peptides (39 to 43 amino acids in length) formed from proteolytic cleavage of the amyloid-beta precursor protein (APP) by the family of secretase enzymes. Although the APP protein is required for a number of biological functions including axonal transport, cell adhesion, and transcription, the amyloid β 1-40 peptide has been studied mostly for its role in neurotoxicity, particularly in the context of Alzheimer’s disease. Previous studies have reported that expression of amyloid β 1-40 increases in Alzheimer’s disease and correlate with the occurrence of aberrant synaptic structure and cognitive dysfunction. These detrimental effects on neural function may be related to aggregation of this peptide (formation of neurite plaques), as neurite plaques been shown to disrupt multiple neurotransmitter systems, and synaptic events such as long-term potentiation.
~4 kDa observed. An uncharacterized band appears at ~19 kDa in some lysates.
Immunogen
Epitope: P3(40) peptide
KLH-conjugated linear peptide corresponding to the P3(40) peptide of human Amyloid, B 1-40.
Application
Anti-Amyloid, β 1-40, aβ Antibody is an antibody against Amyloid, β 1-40, aβ for use in Western Blotting, IHC(P), ELISA.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Amyloid, B 1-40 in human Alzheimer′s diseased brain tissue.
ELISA: A representative lot detected Amyloid, B 1-40 in ELISA (Direct).
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Biochem/physiol Actions
This antibody recognizes the P3(40) peptide of Amyloid, β 1-40.
Physical form
Affinity purified
Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Human Alzhiemer brain tissue lysate
Evaluated by Western Blot in human Alzheimer brain tissue lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Amyloid, B 1-40 on 10 µg of human Alzheimer brain tissue lysate.
Other Notes
Replaces: AB5737
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 100 |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human |
| species reactivity (predicted by homology) | bovine (based on 100% sequence homology), rat (based on 100% sequence homology), mouse (based on 100% sequence homology) |
| technique(s) | ELISA: suitable,immunohistochemistry: suitable (paraffin),western blot: suitable |
| NCBI accession no. | NP_001129601 |
| UniProt accession no. | P05067 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... APP(351) |
