General description
Nucleolar protein 3 (NOL3), also known as Apoptosis repressor with CARD or Muscle-enriched cytoplasmic protein (Myp) or Nucleolar protein of 30 kDa (Nop30) and encoded by the gene name NOL3 or ARC contains one CARD domain. Apoptosis is regulated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. CARD domain containing cell death regulators include RAIDD, Apaf-1, caspase-9, and caspase-2. A novel CARD domain containing protein was recently identified and designated ARC for apoptosis repressor with CARD. ARC interacts with caspase-2 and –8 and inhibits enzymatic activity of caspase-8. ARC suppresses apoptosis induced by cell death adapters FADD and TRADD and by cell death receptors Fas, TNFR-1 and DR3. The messenger RNA of ARC is primarily expressed in skeletal muscle and cardiac tissue.
~24 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Immunogen
Epitope: C-terminus
KLH-conjugated linear peptide corresponding to amino acids 191-208 at the C-terminus of human ARC.
Application
Anti-ARC Antibody detects level of ARC & has been published & validated for use in ARC.
Immunohistochemistry Analysis: 5 μg/mL from a representative lot detected ARC in human heart and skeletal muscle tissues.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Physical form
Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in HeLa, KB, and A549 cell lysates.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ARC in HeLa, KB, and A549 cell lysates.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human |
| technique(s) | immunohistochemistry: suitable,western blot: suitable |
| UniProt accession no. | O60936 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... NOL3(8996) |
