General description
mTOR (Mammalian Target of Rapamycin, aka FRAP, RAPT or RAFT) is a large 289 kDa Ser/Thr protein kinase that regulates cell cycle progression, cell growth, protein synthesis, ribosome biogenesis, and autophagy. mTOR is an evolutionarily conserved member of the Phosphoinositol Kinase-related Kinase (PIKK) family whose activity is regulated by phosphorylation on Ser2448 by Akt in response to insulin or muscle activity. mTORC1 (mTOR Complex 1) consists of at least mTOR, Raptor, and G (mLST8). mTORC1 is known to play a central role in insulin signaling, which is crucial in maintaining metabolic homeostasis. mTORC2 affects cell proliferation and survival primarily by phosphorylating the hydrophobic motif of Akt on Ser473, a well-known effecter of the PI3 Kinase pathway. In addition to phosphorylating Akt, the mTORC2 complex is also known to effect cytoskeletal organization and migration by exerting its effects through Rac, Rho, and PKC. Defects in both mTOR complexes are associated with a variety of diseases, including cancer and diabetes.
~ 289 kDa observed
Immunogen
GST-tagged recombinant protein corresponding to rat mTOR.
Application
Research Sub Category
PI3K, Akt, & mTOR Signaling
This Anti-mTOR Antibody, clone F11 is validated for use in WB, IP for the detection of mTOR.
Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP. (Nemazanyy, I, et al. (2008). Hybridoma, 27:395-398.)
Research Category
Signaling
Physical form
Format: Purified
Protein G
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HeLa cell lysate
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected mTOR on 10 µg of HeLa cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | F11, monoclonal |
| species reactivity | human |
| species reactivity (predicted by homology) | rat (based on 100% sequence homology) |
| technique(s) | immunoprecipitation (IP): suitable,western blot: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NP_063971 |
| UniProt accession no. | P42346 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... MTOR(2475) |
