General description
199.5 kDa Calculated
MAP-2 (microtubule associated protein-2) is one of several high molecular weight proteins that play an important role in brain neuron microtubule assembly. In addition to its association with microtubules, MAP-2 associates with neurofilaments and actin filaments suggesting that it may guide interaction among microtubules, other cytoskeletal elements, and cytoplasmic organelles. MAP-2 is a stringent marker for neurons. In addition, MAP-2 displays intracellular specificity. In the central nervous system, MAP-2 is confined to neuronal cell bodies and dendrites. There are exceptions, however, where some axons stain positive for small amounts of MAP-2. MAP-2 is uniformly distributed throughout the cell when first expressed in cultured neurons but becomes selectively localized as dendritic development proceeds. (Caceres, 1986; Dotti, 1987).
Immunogen
Recombinant Bovine brain microtubule protein.
Application
Milli-Mark Anti-MAP2-PE Antibody, clone AP20 is an antibody against MAP2 for use in FC.
Research Category
Neuroscience
Research Sub Category
Neuronal & Glial Markers
Biochem/physiol Actions
Antibody recognizes MAP-2.
Physical form
Protein A purified
Purified mouse monoclonal IgG1 conjugated to PE in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Preparation Note
Maintain refrigerated at 2-8°C protected from light for up to 6 months from date of receipt.
Analysis Note
Control
U251 cells
Evaluated by flow cytometry using U251 cells
Legal Information
MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | PE |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | AP20, monoclonal |
| species reactivity | human |
| species reactivity (predicted by homology) | bovine (immunogen homology) |
| manufacturer/tradename | Milli-Mark® |
| technique(s) | flow cytometry: suitable |
| isotype | IgG1 |
| NCBI accession no. | NP_002365 |
| UniProt accession no. | P11137 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... MAP2(4133) |
