General description
N-terminal 6His-tagged recombinant human Txk, residues 256-end.
Product Source: Expressed by baculovirus in Sf21 insect cells.
Application
Research Category
Inflammation & Immunology
Biochem/physiol Actions
Protein Target: Txk
Target Sub-Family: TK
Packaging
Also available in 250 µg size --call for pricing and availability and reference catalog number 14-761M when ordering the 250 µg size.
Physical form
50mM Tris-HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Ni2+/NTA-agarose
Preparation Note
Store -70°C. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.
Other Notes
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | human |
| Quality Segment | 100,300 |
| recombinant | expressed in Sf21 cells |
| mol wt | Mw 35.3 kDa |
| manufacturer/tradename | Upstate® |
| technique(s) | activity assay: suitable (kinase) |
| NCBI accession no. | NM_003328 |
| UniProt accession no. | P42681,P42682 (mouse) |
