General description
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen. Recognizes the ~53 kDa wild-type and mutant forms of p53.
Recognizes the ~53 kDa wild-type p53 and mutant p53 protein in SKBR-3 and MDA-231 cells and in colon carcinoma tissue.
This Anti-p53 (Ab-12) (Pantropic) Mouse mAb (DO-7) is validated for use in Frozen Sections, Immunoblotting, IF, IP, Paraffin Sections for the detection of p53 (Ab-12) (Pantropic).
Immunogen
Epitope: within amino acids 37-45 of p53
recombinant, human p53 expressed in E. coli
Application
Frozen Sections (1-2 g/ml)
Immunoblotting (1-2 g/ml)
Immunofluorescence (0.5 g/ml)
Immunoprecipitation (2 g/mg lysate)
Paraffin Sections (1-2 g/ml)
Packaging
Please refer to vial label for lot-specific concentration.
Analysis Note
Positive Control
SKBR-3 or MDA-231 cells or colon carcinoma tissue
Other Notes
Exhibits nuclear staining in tumor cells. Antibody should be titrated for optimal results in individual systems.
Greenblatt, M.S., et al. 1994. Cancer Res.54, 4855.
Lane, D.P. 1992. Nature358, 15.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
| biological source | mouse |
| Quality Level | 100 |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | DO-7, monoclonal |
| form | liquid |
| does not contain | preservative |
| species reactivity | bovine, monkey, human |
| manufacturer/tradename | Calbiochem® |
| storage condition | OK to freeze,avoid repeated freeze/thaw cycles |
| dilution | (Frozen Sections (1-2 µg/mL) Immunoblotting (1-2 µg/mL) Immunofluorescence (0.5 µg/mL) Immunoprecipitation (2 µg/mg lysate) Paraffin Sections (1-2 µg/mL)) |
| isotype | IgG2b |
| shipped in | wet ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... TP53(7157) |
