Immunogen
Epitope: Complete sequence
Recombinant full length rat NAP-22.
Application
Immunoblotting: 1:1,000 using rat brain extract (colormetric; higher dilutions may be used with ECL). The antibody reacts with the 55 kDa NAP-22 protein. The suggested dilution buffer is TBS containing 0.05% Tween-20. Suggested blocking buffer is 5% non-fat dry milk. Suggested transfer membrane is PVDF-Immobilon. Suggested gel percentage is 10%. Overnight incubation with the antibody is recommended.
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience
Neuroregenerative Medicine
This Anti-NAP-22 Antibody, complete sequence is validated for use in WB for the detection of NAP-22.
Biochem/physiol Actions
Recognizes NAP-22 [Neuronal acidic protein 22, CAP-23, BASP1].
Physical form
Format: Purified
Semi-purified immunoglobulin. Liquid in TBS containing 0.05% sodium azide.
Preparation Note
Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
Analysis Note
Control
POSITIVE CONTROL: Neonatal rat brain extract. NAP-22 is heat and acid resistant. Heat or acid-treated supernatants from crude tissue or cell homogenates are good sources to analyze NAP-22 content by immunoblot.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | rat |
| manufacturer/tradename | Chemicon® |
| technique(s) | western blot: suitable |
| NCBI accession no. | NM_006317.3 |
| UniProt accession no. | P80723 |
| shipped in | dry ice |
| target post-translational modification | unmodified |
| Gene Information | rat ... Basp1(64160) |
