General description
The vasoactive intestinal peptide/pituitary adenylate cyclase activating peptide receptor 1 (VPAC1) is a member of a class II subfamily of G-protein-coupled receptors and has a high affinity for vasoactive intestinal peptide (VIP). VPAC1 has been associated with many different lung cancer cell lines. VPAC1 is thought to be activated by FXR and may contribute to the regulation of bile secretion to protect against gallstone disease. VPAC1 could be used to regulate VIPs and, in turn, inhibit breast cancer growth. VPAC1 has also been implicated in regulation of the anti-inflammatory effects of VIP.
~52 kDa observed. Multiple molecular weights of VPAC1 may be due to glycosylation. (Fabre, C, et al. (1993). Peptides. 14:483-489.)
Immunogen
Epitope: Cytoplasmic domain
KLH-conjugated linear peptide corresponding to the cytoplasmic domain of VPAC1.
Application
Anti-VPAC1 Antibody detects level of VPAC1 & has been published & validated for use in IH(P) & WB.
Immunohistochemistry Analysis: 1:500 dilution from a previous lot detected VPAC1 in ovarian carcinoma tissue.
Research Category
Neuroscience
Research Sub Category
Hormones & Receptors
Biochem/physiol Actions
This antibody recognizes VPAC1 at the cytoplasmic domain.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
SK-OV-3 cell lysate
Evaluated by Western Blot in SK-OV-3 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected VPAC1 on 10 µg of SK-OV-3 cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | mouse, human, rat |
| technique(s) | immunohistochemistry: suitable (paraffin),western blot: suitable |
| NCBI accession no. | NP_035833 |
| UniProt accession no. | P97751 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... VIPR1(7433) |
