General description
Histone H3 is one of the five main histone proteins that are involved in the structure of chromatin in eukaryotic cells. Histone H3 features a main globular domain and a long N-terminal tail. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 is specifically phosphorylated during both mitosis and meiosis in patterns that are specifically coordinated in space and time. These phosphorylations are initiated at different phases of the cell division. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.
~16 kDa observed
Immunogen
Epitope: Trimethylated (Lys4)
Synthetic peptide corresponding to residues surrounding and including trimethlyated Lys 4 of Histone H3.
Application
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected trimethyl-Histone H3 (Lys4) in NIH/3T3 cells.
The unconjugated version of this antibody has been shown to work in ChIP, DB, WB, ChIP-seq.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
This Anti-trimethyl-Histone H3 (Lys4) Antibody, Alexa Fluor™ 647 Conjugate is validated for use in Immunocytochemistry for the detection of trimethyl-Histone H3.
Biochem/physiol Actions
Expected to react with most other species based on extreme conserved homology among species.
Physical form
Affinity purified
Purified rabbit conjugate antibody in buffer containing PBS with 15 mg/ml BSA and 0.1% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Immunocytochemistry in A431 cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected trimethyl-Histone H3 (Lys4) in A431 cells.
Other Notes
Concentration: Please refer to lot specific datasheet.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | ALEXA FLUOR™ 647 |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | human, mouse |
| species reactivity (predicted by homology) | vertebrates (based on 100% sequence homology) |
| technique(s) | immunocytochemistry: suitable |
| NCBI accession no. | NM_003493 |
| UniProt accession no. | Q16695 |
| shipped in | wet ice |
| target post-translational modification | trimethylation (Lys4) |
| Gene Information | human ... H3C1(8350) mouse ... H3C1(360198) |
