General description
Programmed cell death 6-interacting protein (UniProt: Q8WUM4; also known as PDCD6-interacting protein, PDCD6IP, ALG-2-interacting protein 1, ALG-2-interacting protein X, Hp95) is encoded by the PDCD6IP (also known as AIP1, ALIX, KIAA1375) in human. PDCD6IP is a multifunctional protein that interacts with PDCD6 in a calcium-dependent manner. It is involved in endocytosis, multivesicular body biogenesis, membrane repair, cytokinesis, apoptosis and maintenance of tight junction integrity. It is a class E VPS protein that is involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. It also serves as an adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. It is reported to play a role in the regulation of both apoptosis and cell proliferation and its overexpression blocks apoptosis. Three isoforms of PDCD6IP have been described that are produced by alternative splicing.
~96 kDa observed; 96.02 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Immunogen
GST/His-tagged recombinant fragment corresponding to 126 amino acids from human Programmed cell death 6-interacting protein (PDCD6).
Application
Anti-PDCD6-interacting protein, clone 12H1.2, Cat. No. MABT1229 is a mouse monoclonal antibody that detects Programmed cell death 6-interacting protein and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Western Blotting Analysis: 1 µg/mL from a representative lot detected PDCD6-interacting protein in Jurkat cell lysate.
Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected PDCD6-interacting protein in human pancreas, human prostate cancer, and human cervical cancer tissues.
Biochem/physiol Actions
Clone 12H2.1 detects Programmed cell death 6-interacting protein in human cells. It targets an epitope within 126 amino acids from the C-terminal region.
Physical form
Format: Purified
Analysis Note
Evaluated by Western Blotting in TF-1 cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected PDCD6-interacting protein in TF-1 cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 12H2.1, monoclonal |
| species reactivity | human |
| packaging | antibody small pack of 25 μg |
| technique(s) | immunohistochemistry: suitable (paraffin),western blot: suitable |
| isotype | IgG2aκ |
| NCBI accession no. | NP_037506 |
| UniProt accession no. | Q8WUM4 |
| shipped in | ambient |
| target post-translational modification | unmodified |
| Gene Information | human ... PDCD6IP(10015) |
