General description
Peptide transporter TAP1 is also called Antigen peptide transporter 1 (APT1), ATP-binding cassette sub-family B member 2 (ABCB2), Peptide supply factor 1 (PSF1), Peptide transporter PSF1 (PSF-1), Peptide transporter involved in antigen processing 1, and Really interesting new gene 4 protein (RING4). TAP1 is involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules as well as MHC class I folding. TAP1 is inhibited herpes simplex virus ICP47 protein, human cytomegalovirus US6 glycoprotein, human adenovirus E3-19K glycoprotein, and is down-regulated by human Epstein-Barr virus vIL-10 protein. TAP1 mutations can be a cause of Bare lymphocyte syndrome 1 (BLS1).
~65 kDa observed. Uniprot describes a molecular weight of ~87 kDa However, this protein may be observed at ~65 kDa (Koch, J., et al. (2006). FEBS Lett. 580(17):4091-4096.).
Immunogen
Epitope: This antibody recognizes the ADAPE amino acid residues within the CYWAMVQAPADAPE sequence of human TAP1 (located at the C-terminus).
Linear peptide corresponding to the CYWAMVQAPADAPE sequence of human TAP1.
Application
Detect Antigen peptide transporter 1 using this mouse monoclonal antibody, Anti-TAP1 Antibody, clone mAb 148.3 validated for use in western blotting, IP, Immunofluorescence & Activity Assay.
Western Blotting Analysis: A representative lot from an independent laboratory detected TAP1 in transiently transfected HeLa cells (Hulpke, S., et al. (2012). FASEB J. 26(12):5071-5080.).
Western Blotting Analysis: A representative lot from an independent laboratory detected TAP1 in microsomes of baculovirus-infected SF9 cells, which express wild type or select mutations of TAP1 (Chen, M., et al. (2004). J Biol Chem. 279(44):46073-46081.).
Western Blotting Analysis: A representative lot from an independent laboratory detected TAP1 in SF9 cells infected with recombinant baculovirus containing TAP1 gene constructs (Meyer, T. H., et al. (1994). FEBS Lett. 351(3):443-447.).
Immunofluorescence Analysis: A representative lot from an independent laboratory detected TAP1 in HeLa cells contransfected with wild type TAP1 and TAP2 (Hulpke, S., et al. (2012). Cell Mol Life Sci. 69(19):3317-3327.).
Immunofluorescence Analysis: A representative lot from an independent laboratory detected TAP1 in SF9 cells infected with rBV-TAP1/rBV-TAP2 (Meyer, T. H., et al. (1994). FEBS Lett. 351(3):443-447.).
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated TAP1 from SF9 cells infected with rBV-TAP1/rBV-TAP2 (Meyer, T. H., et al. (1994). FEBS Lett. 351(3):443-447.).
Activity Assay Analysis: This antibody inhibits TAP-specific peptide transport (Plewnia, G., et al. (2007). J Mol Biol. 369(1):95-107.).
Physical form
Format: Purified
Purified mouse monoclonal IgG1κ supernatant in buffer containing PBS without preservatives.
Analysis Note
Evaluated by Western Blotting in untreated and Interferon-gamma (IFN-g) treated HeLa cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected TAP1 in 10 µg of Interferon-gamma (IFN-g) treated HeLa cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
| biological source | mouse |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | mAb 148.3, monoclonal |
| species reactivity | human |
| technique(s) | activity assay: suitable,immunofluorescence: suitable,immunoprecipitation (IP): suitable,western blot: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NP_000584 |
| UniProt accession no. | Q03518 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... TAP1(6890) |
