General description
Sphingosine 1-phosphate receptor 1(S1PR1 or S1P1), otherwise known as EDG1, is a G-protein coupled receptor which is activated by lysosphingolipid sphingosine 1-phosphate (S1P) and lysophosphatidic acid (LPA). It is coupled to Gi-protein and MAP kinase signaling pathways, and may cooperate with other S1P receptors to regulate various cellular processes such as differentiation, actin remodeling, and migration. S1P1 is expressed in epithelial cells, fibroblasts, melanocytes, and smooth muscle cells.
~40 kDa observed
Immunogen
GST-tagged recombinant protein corresponding to mouse Sphingosine 1-phosphate receptor 1 (S1P1).
Application
Research Category
Apoptosis & Cancer
Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling
This Anti-Sphingosine 1-phosphate receptor 1 (S1P1) Antibody, clone 8B7.1 is validated for use in Western Blotting, IHC for the detection of Sphingosine 1-phosphate receptor 1 (S1P1).
Western Blot Analysis: A 1:500 dilution from a representative lot detected Sphingosine 1-phosphate receptor 1 (S1P1) in human brain tissue lysate.
Immunohistochemistry Analysis: A 1:300 dilution from a representative lot detected Sphingosine 1-phosphate receptor 1 (S1P1) in rat choroid plexus tissue.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2a supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Mouse brain tissue lysate
Evaluated by Western Blot in mouse brain tissue lysate.
Western Blot Analysis: A 1:1,000 diluiton of this antibody detected Sphingosine 1-phosphate receptor 1 (S1P1) in 10 µg of mouse brain tissue lysate.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 8B7.1, monoclonal |
| species reactivity | mouse, human, rat |
| technique(s) | immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG2a |
| NCBI accession no. | NP_001391 |
| UniProt accession no. | P21453 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... S1PR1(1901) |
