General description
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Histone proteins are highly post-translationally modified, and Histone H3 is the most extensively modified of the five histones. The term "Histone H3" alone is purposely ambiguous in that it does not distinguish between sequence variants or modification state. Histone H3 is an important protein in the emerging field of epigenetics, where its sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes.
~17 kDa
Immunogen
Epitope: Dimethyl (Lys9) & Phospho (Ser10)
Synthetic linear peptide corresponding to dimethyl (Lys9) and Phospho (Ser10) of Histone H3.
Application
Immunocytochemistry:
A 1:500 dilution of a representative lot of this antibody detected Dimethyl (Lys9)-Phospho (Ser10) Histone H3 in A431 and HeLa cells.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
Use Anti-Histone H3 Antibody (Dimethyl K9, Phospho S10), clone 6HH3-2C5 (mouse monoclonal antibody) validated in WB, ICC to detect Histone H3 (Dimethyl K9 also known as H3K9me2S10P, Histone H3 (di methyl K9 phospho S10).
Biochem/physiol Actions
Antibody recognizes dimethyl (Lys9) phospho(Ser10) on Histone H3.
Physical form
Mouse monoclonal IgG1κ ascites with 0.05% sodium azide.
Unpurified
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
Untreated and Etoposide-treated HeLa acid extracts.
Evaluated by western blot in untreated and Etoposide-treated HeLa acid extracts.
Western Blot Analysis:
A 1:1,000 dilution of this antibody detected Dimethyl (Lys9)-Phospho (Ser10) Histone H3 in 10 µg of untreated and Etoposide-treated HeLa acid extracts.
Legal Information
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Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| clone | 6HH3-2C5, monoclonal |
| species reactivity | human |
| species reactivity (predicted by homology) | Drosophila (based on 100% sequence homology) |
| technique(s) | immunocytochemistry: suitable,western blot: suitable |
| isotype | IgG1κ |
| GenBank accession no. | AK304607 |
| UniProt accession no. | B4E380 |
| shipped in | wet ice |
| target post-translational modification | dimethylation (Lys9), phosphorylation (pSer10) |
| Gene Information | human ... H3F3B(3021) |
