General description
34 kDa (glycosylated)
Erythropoietin (Epo) is a glycoprotein consisting of one 165-amino acid single-chain polypeptide with two internal disulfide bonds. When fully glycosylated, the molecular weight of Epo is approximately 34 kDa. Normally produced by the liver and kidney, Epo is the principle regulator of red blood cell production by stimulating the division and differentiation of bone marrow erythroid progenitor cells in response to anemia and hypoxia.
Immunogen
Recombinant human Epo.
Application
Detect Erythropoietin using this Anti-Erythropoietin Antibody, clone 1C8 validated for use in ELISA, IF & WB.
ELISA:
For the determination of Epo concentration in the range of 2-200 ng/mL. Use at 5 ug/mL to coat plate.
Immunosorbent preparation
Optimal working dilutions must be determined by the end user.
Research Category
Metabolism
Research Sub Category
Metabolic Hormones & Receptors
Biochem/physiol Actions
Reacts with human Erythropoietin (Epo).
Physical form
Format: Purified
Protein A purified
Purified mouse monoclonal IgG2b in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4.
Preparation Note
Stable for 1 year at 2-8ºC from date of receipt.
Analysis Note
Control
Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.
Evaluated by Western Blot on Mouse kidney lysates.
Western Blot Analysis:
1:1000 dilution of this antibody detected glycosylated EPO on 10 µg of Mouse kidney lysates.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| clone | 1C8, monoclonal |
| species reactivity | human |
| manufacturer/tradename | Chemicon® |
| technique(s) | ELISA: suitable,immunofluorescence: suitable,western blot: suitable |
| isotype | IgG2b |
| NCBI accession no. | NM_000799.2 |
| UniProt accession no. | P01588 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
