General description
M.W. 23,000 (monomer); 115,000 (pentamer).
Recombinant, human C-reactive protein expressed in E. coli. CRP is a major acute-phase plasma protein that is dramatically elevated in patients with acute-phase conditions. Composed of five identical noncovalently linked subunits. Exhibits Ca2+-dependent binding to a variety of substrates. Important in diagnosis of rheumatoid arthritis. Suitable for immunological studies. In purified states and in plasma the monomeric form (M.W. ~21 kDa) aggregates as a cyclic pentamer. Intended for use as a calibration standard for assays of CRP, for structural and functional studies, and as an antigen for production of antisera.Calibrated by electroimmunoassay against WHO reference standard or equivalent.
Physical form
In 140 mM NaCl, 20 mM Tris-HCl, 2 mM CaCl₂, 0.05% NaN₃, pH 7.5.
Other Notes
Paffen, E., et al. 2004. Arterioscler. Thromb. Vasc. Biol.24, 975.
Ballou, S.P., and Kushner, I. 1992. Adv. Intern. Med. 37, 313.
Stults, N.L., et al. 1987. Anal. Biochem. 161, 567.
Woo, P., et al. 1985. J. Biol. Chem. 260, 13384.
de Beer, F.C., and Pepys, M.B. 1982. J. Immun. Methods 50, 17.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Toxicity: Standard Handling (A)
| recombinant | expressed in E. coli |
| Quality Level | 100 |
| description | Merck USA index - 14, 2603 |
| form | liquid |
| manufacturer/tradename | Calbiochem® |
| storage condition | do not freeze |
| solubility | aqueous buffer: soluble |
| storage temp. | 2-8°C |
