General description
MSH3, also known as DNA mismatch repair protein Msh3, Protein repair-1, REP-1, or Protein repair-3, REP-3, and encoded by the gene MSH3/REP-3, is an important component of the post-replicative DNA mismatch repair system. MSH3 heterodimerizes with MSH2 to form a complex named MutS beta which binds DNA mismatches and initiates DNA repair. MSH3/MSH2 complex (MutS beta) also complexes with another protein complex called MutL alpha which is also necessary for further steps in DNA repair. MSH3 is also frequently mutated in many cancers resulting in a mismatch repair deficiency and this can contribute to sensitivity to cytotoxic drugs. Interestingly too, because MSH3 is involved in DNA repair, it appears to it can mediate sensitivity to various repeat-associated diseases such as Huntington’s disease.
~123 kDa observed
Immunogen
Recombinant protein corresponding to mouse MSH3.
Application
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
This Anti-MSH3 Antibody, clone 1F6 is validated for use in Western Blotting and Immunocytochemistry for the detection of MSH3.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected MSH3 in 10 µg of mouse testis tissue lysate.
Immunocytochemistry Analysis: A representative lot detected MSH3 in C2C12 cells (Prof. Glenn Morris, Wolfson Centre for Inherited Neuromuscular Disease.; Holt, I., et al. (2011). J Cell Biochem. 112(6):1612-1621.).
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in C2C12 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected MSH3 in 10 µg of C2C12 cell lysate.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 1F6, monoclonal |
| species reactivity | mouse |
| concentration | 1 mg/mL |
| technique(s) | immunocytochemistry: suitable,western blot: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NP_034959 |
| UniProt accession no. | P13705 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | mouse ... Msh3(17686) |
