Immunogen
Synthetic 30 amino acid peptide corresponding to amino acids 383-412 of the canine adenosine A2a receptor near the C-terminal intracellular domain (30 amino acids) of the cloned canine A2a receptor. The A2a sequence selected in not present in A1, A2b and A3 receptors.
Application
This Anti-Adenosine A2a Receptor Antibody is validated for use in ELISA, WB for the detection of Adenosine A2a Receptor.
Western blot: 1:1,000-1:5,000 (Chemiluminescence technique), 40-100 μg membrane proteins/lane of striatal membranes. Higher concentrations of the antibody may be necessary if colorimetric detection or milk-based antibody diluent are used.
ELISA: 1:10,000-1:50,000 (50-100 ng A2aR peptide (Cat. Number AG285)/well)
Optimal working dilutions must be determined by end user.
Physical form
Rabbit antiserum. Liquid containing 0.1% sodium azide.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
| biological source | rabbit |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | serum |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | pig, bovine, rat, human, canine |
| manufacturer/tradename | Chemicon® |
| technique(s) | ELISA: suitable,western blot: suitable |
| suitability | not suitable for immunohistochemistry |
| NCBI accession no. | NM_000675.4 |
| UniProt accession no. | P29274 |
| shipped in | dry ice |
| target post-translational modification | unmodified |
| Gene Information | human ... ADORA2A(135) |
