Immunogen
Murine NIH 3T3 cells stably transfected with the human (6 subunit
Application
Anti-Integrin β6 Antibody, clone R6G9, azide free is an antibody against Integrin β6 for use in FC & IP.
Flow cytometry
Immunoprecipitation: Effective; however because this antibody is an IgG2a, protein G or rabbit anti-mouse IgG is helpful for capture.
Immunohistochemistry/immunocytochemistry: The antibody recognizes alphaVbeta6 by immunohistochemistry and immunocytochemistry (acetone fix), but nuclear background staining limits usefulness in these techniques.
Then antibody is not function blocking and not effective for western blot.
Optimal working dilutions must be determined by end user.
Biochem/physiol Actions
Monoclonal antibody MAB2075Z, raised by immunizing Balb/c mice with murine NIH 3T3 cells stably transfected with the human beta6 subunit, recognizes the human beta6 subunit by flow cytometry and is an excellent antibody for immunoprecipitation.
Physical form
Format: Purified
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
| biological source | mouse |
| Quality Segment | 100 |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | R6G9, monoclonal |
| species reactivity | human |
| manufacturer/tradename | Chemicon® |
| technique(s) | flow cytometry: suitable,immunoprecipitation (IP): suitable |
| isotype | IgG2a |
| suitability | not suitable for Western blot,not suitable for activity/function inhibition |
| NCBI accession no. | NM_000888.3 |
| UniProt accession no. | P18564 |
| shipped in | dry ice |
| target post-translational modification | unmodified |
| Gene Information | human ... ITGB6(3694) |
