产品介绍
Product Description
General description
抗CD3,小鼠单克隆,克隆UCHT1,可识别人外周T细胞中CD3复合物的ε-链。已针对冰冻切片的FC,IP,IHC和T细胞共刺激进行了验证。
蛋白A纯化的小鼠单克隆抗体。识别CD3 T细胞受体复合物的~20 kDaε链。
识别人外周T细胞中CD3复合物的ε-链。可用于激活表达CD3ε的T细胞。
Immunogen
人胸腺细胞/Sezary T细胞
Application
流式细胞术(5 µg/ml)
冷冻切片(参见备注)
免疫沉淀法(参见备注)
T细胞共刺激(参见应用参考)
Packaging
请参考特定浓度批号的标签。
Other Notes
Thibault, G. and Bardos, P. 1995.J. Immunol.154, 3814.
Beverley, P.C., and Callard, R.E.1981.Eur. J. Immunol.11, 329.
该抗体可用于激活表达CD3ε的T细胞(见应用参考)。对5×105培养的HPB-MLT人肿瘤细胞进行FACS。当与二级试剂山羊抗小鼠IgG(FITC偶联)一起使用时,测试的**结合浓度为5 µg/mL。据报道,该抗体可用于免疫沉淀和冷冻切片染色。合适的工作稀释度由与测定条件相关的变量决定。
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
毒性:标准处理(A)
技术参数
Specifications
| biological source | mouse |
| Quality Level | 100 |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | UCHT1, monoclonal |
| assay | >95% (immunoglobulin, SDS-PAGE) |
| form | liquid |
| contains | 0.5 mg/mL gentamycin sulfate as preservative |
| species reactivity | human |
| manufacturer/tradename | Calbiochem® |
| storage condition | OK to freeze |
| dilution | (Flow Cytometry (5 µg/mL) Frozen Sections Immunoprecipitation T cell Co-stimulation ) |
| isotype | IgG1 |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |

