General description
Polymerase I and transcript release factor (PTRF), also known as “cavin-1,” is a small cytoplasmic protein, which interacts with caveolin-1 to assemble specialized membrane invaginations (or caveolae), in various types of cells. Caveolae house specific proteins and lipids and play a central role in cell signaling and endocytosis. PTRF may also play a role in metabolism. Mice lacking functional PTRF are affected by hyperinsulinemia and glucose intolerance. In addition, PTRF may play a role in prostate cancer.
~60 kDa, ~43 kDa observed. Three isoforms at 43 kDa, 33 kDa, and 23 kDa are known to exist. This protein was observed at ~60 kDa by an independent laboratory due to post-translational modification (Hill, M. M., et al. (2008). Cell. 132(1):113-124.).
Immunogen
KLH-conjugated linear peptide corresponding to the Coiled Coil domain of human PTRF/cavin-1.
Application
Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected PTRF/cavin-1 in mouse cardiac tissue and mouse alveoli tissue.
Anti-PTRF/cavin-1 Antibody detects level of PTRF/cavin-1 & has been published & validated for use in Western Blotting, IHC(P).
Biochem/physiol Actions
This antibody recognizes the Coiled Coil domain of PTRF/cavin-1.
Analysis Note
Evaluated by Western Blot in human lung tissue lysate.
Western Blot Analysis: 1 mg/mL of this antibody detected PTRF/cavin-1 in 10 mg of human lung tissue lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | mouse, rat, human |
| species reactivity (predicted by homology) | bovine (based on 100% sequence homology) |
| technique(s) | immunohistochemistry: suitable (paraffin),western blot: suitable |
| NCBI accession no. | NP_036364 |
| UniProt accession no. | Q6NZI2 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... CAVIN1(284119) |
