General description
~40 kDa observed
MBNL2, also known as Muscleblind-like protein 2, Muscleblind-like protein 1, Muscleblind-like protein-like, or Muscleblind-like protein-like 39, and encoded by the gene MBNL2/MBLL/MBLL39/MLP1, is a protein that mediates pre-mRNA alternative splicing regulation. MBNL2 acts either as an activator or repressor of splicing on specific pre-mRNA targets. For instance MBNL2 inhibits cardiac troponin-T pre-mRNA exon inclusion but induces insulin receptor pre-mRNA exon inclusion in muscle. MBNL2 may also regulate ITGA3 expression by transporting it from the nucleus to cytoplasm at adhesion plaques. MBNL2 appears to play a key role in the muscle disease named myotonic dystrophy pathophysiology. MBNL2 is expressed in the nucleus of cells in multiple tissues but can be found in the cytoplasm in certain situations. In particular it is found in the cytoplasm in adhesion plaques.
Immunogen
Recombinant protein corresponding to human MBNL2.
Application
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
This Anti-MBNL2 Antibody, clone MB2a(3B4) is validated for use in Western Blotting for the detection of MBNL2.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in C2C12 cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected MBNL2 in 10 µg of C2C12 cell lysate.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | MB2a(3B4), monoclonal |
| species reactivity | mouse |
| species reactivity (predicted by homology) | human (based on 100% sequence homology) |
| concentration | 1 mg/mL |
| technique(s) | western blot: suitable |
| isotype | IgG2bκ |
| NCBI accession no. | NP_659002 |
| UniProt accession no. | Q5VZF2 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... MBNL2(10150) |
