General description
165 kDa (Wright, 1988)
CD11b is the 165 kDa α-chain of the Mac-1 complex. The molecule, also called CR3, functions as a cell surface receptor for the C3bi complement fragment. There is evidence that CD11b can bind fibrinogen in addition to C3bi (Wright,1988). Two binding sites have now been reported, one recognizing lipopolysaccharide, and the other protein ligands containing the arg-gly-asp sequence (Wright, 1989). Binding of the coagulation factor X has also been suggested as an additional receptor role of CD11b (Altieri,1988). This protein belings to a family of related heterodimeric proteins designated CD11 in the classification system for human leucocyte differentiation antigens, which shares a common β-chain with a MW of 95 kDa (CD18). Besides the C3bi receptor, this protein family contains two other members, leucocyte function associated-1 (LFA-1, C11a) antigen and p150,95 protein (CD11c) (Sanchez-Madrid,1983).
Immunogen
Purified iC3b receptor.
Application
Milli-Mark Anti-CD11B-PE Antibody, clone 2LPM19c detects level of CD11B & has been published & validated for use in FC.
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
Biochem/physiol Actions
Antibody recognizes Human CD11b antigen.
Physical form
Protein A purified
Purified Mouse monoclonal IgG1 conjugated to PE in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2.
Preparation Note
Maintained refrigerated at 2-8°C from date of receipt. Protected from light in undiluted aliquots for 6 months from date of receipt.
Analysis Note
Control
PBMCs
Evaluated by flow cytometry using human PBMCs
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Level | 100 |
| conjugate | PE |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 2LPM19c, monoclonal |
| species reactivity | human |
| manufacturer/tradename | Milli-Mark® |
| technique(s) | flow cytometry: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NP_000623.2 |
| UniProt accession no. | P11215 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... ITGAM(3684) |
