General description
CTCF, also known as CCCTC-binding factor, contains 11 zinc finger (ZF) binding domains that allow it to bind different DNA target sequences and proteins. This multifunctional protein can act as a transcriptional activator or repressor. As a transcriptional repressor, CTCF, binds to promoters of vertebrate c-myc gene. It also binds to the PLK and PIM1 promoters. CTCF may prevent the access of transcriptional activators to enhancers. CTCF also acts as a transcriptional activator of APP. It is involved in different aspects of gene regulation including promoter activation or repression, hormone-responsive gene silencing, methylation-dependent chromatin insulation, and genomic imprinting. Mutations of CTCF in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms tumors, suggesting a role for CTCF may as a tumor suppressor.
~ 130 kDa
Note: Poly(ADP-ribosyl)ated isoform, C-terminal truncation, or sumoylation will cause variable migration of CTCF protein on SDS–PAGE, which may present CTCF with different molecular weight on Western Blot. Modifications are dependent on cell type and growing condition.
The following molecular weights have been reported in the literature: 180 kDa (CTCF-180), 130 kDa (CTCF-130), 82 kDa (CTCF-82), 70 kDa (CTCF-70). See Klenova,E.M.,et al, (1997) Nucleic Acids Res. 25, 466–473 and Torrano,V,et al, (2006) Journal of Cell Science 119, 1746-1759) for additional details.
Immunogen
His-tagged recombinant protein corresponding to human CTCF.
Application
Anti-CTCF Antibody, clone 1.2.1.5.4 is a Mouse Monoclonal Antibody for detection of CTCF also known as 11-zinc finger protein, CCCTC-binding factor & has been validated in WB, IP & IHC.
Chromatin Immunoprecipitation Analysis:
A representative lot was used by an independent laboratory in ChIP. (Pugacheva, E., et al. (2005). Human Molecular Genetics. 14(7):953–965.)
Biochem/physiol Actions
This antibody recognizes CTCF.
Physical form
Format: Purified
Analysis Note
Evaluated by Western Blot in K562 cell lysate.
Western Blot Analysis: 1 µg/ml of this antibody detected CTCF on 10 µg of K562 cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: MABE547
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 1.2.1.5.4, monoclonal |
| species reactivity | human |
| technique(s) | ChIP: suitable,immunohistochemistry: suitable,immunoprecipitation (IP): suitable,western blot: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NP_006556.1 |
| UniProt accession no. | P49711 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... CTCF(10664) |
