General description
PHD finger protein 1 (UniProt: O43189; also known as PHF1, hPHF1, Polycomb-like protein 1, hPCl1) is encoded by the PHF1 (also known as PCL1) gene (Gene ID: 5252) in human. PHF1 is a member of the polycomblike family and contains two PHD-type zinc fingers (aa 87-142 and 186-240) and one Tudor domain (aa 29-86). It is essential for epigenetic regulation and genome maintenance. It specifically binds histone H3 trimethylated at ′Lys-36′ (H3K36me3) via its Tudor domain and recruits the PRC2 complex. PHF1 is highly expressed in heart, skeletal muscle, and pancreas, with lower levels found in brain, placenta, lung, liver and kidney. It is involved in DNA damage response and is recruited at double-strand breaks. PHF1 localizes to the promoters of numerous target genes and to double-strand breaks (DSBs) sites following DNA damage. It has been reported that PRC2 recruitment by PHF1 promotes H3K27me3 and subsequent gene silencing by inducing spreading of PRC2 and H3K27me3 into H3K36me3 loci. A chromosomal aberration involving PHF1 is reported to be the causative factor in endometrial stromal tumors. (Ref.: Sarma, K et al. (2008). Mol. Cell. Biol. 28(8): 2718-31; Musselman, CA et al (2012). Nat. Struct. Mol. Biol. 19(12):1266-72).
~63 kDa observed; 62.11 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Immunogen
GST/His-tagged recombinat fragment corresponding to 137 amino acids from human PHD finger protein 1.
Application
Anti-PHF1, clone 12C2.1, Cat. No. MABE676, is a highly specific mouse monoclonal antibody that targets PHF1 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected PHF1 in human esophagus and human tonsil tissue.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PHF1 in 10 µg of HeLa cell lysate.
Research Category
Epigenetics & Nuclear Function
Biochem/physiol Actions
Clone 12C2.1 specifically detects PHF1 in human tissues. It targets an epitope within 137 amino acids from the internal region.
Physical form
Format: Purified
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in mouse heart tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PHF1 in 10 µg of mouse heart tissue lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 12C2.1, monoclonal |
| species reactivity | human, mouse |
| technique(s) | immunohistochemistry: suitable (paraffin),western blot: suitable |
| isotype | IgG2bκ |
| NCBI accession no. | NP_002627 |
| UniProt accession no. | O43189 |
| shipped in | ambient |
| target post-translational modification | unmodified |
| Gene Information | human ... PHF1(5252) |
