General description
Ubiquitin-conjugating enzyme E2 A (EC 6.3.2.19; UniProt P49459; also known as hHR6A, HR6A, RAD6 homolog A, Ubiquitin carrier protein A) is encoded by the UBE2A (also known as MRXS30, MRXSN, RAD6A, UBC2) gene (Gene ID 7319) in human. UBE2A accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. UBE2A is involved in transcription regulation by catalyzing the monoubiquitination of histone H2B Lys120 to form H2BK120ub1. H2BK120ub1 serves as a specific signal for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, as well as a prerequisite for H3K4me and H3K79me formation. In vitro, UBE2A catalyzes both Lys11 and Lys48-linked polyubiquitination. UBE2A is also involved in UV-damaged DNA repair and aberrant UBE2A can cause mental retardation syndromic X-linked Nascimento-type (MRXSN).
~17 kDa observed. Uncharacterized band(s) may appear in some lysates.
Immunogen
Epitope: Near C-terminus
KLH-conjugated linear peptide corresponding to human Ubiquitin-conjugating enzyme E2 A sequence near the C-terminus.
Application
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
This Anti-Ubiquitin-conjugating enzyme E2 A Antibody is validated for use in Western Blotting for the detection of Ubiquitin-conjugating enzyme E2 A.
Biochem/physiol Actions
Expected to react with all three spliced isoforms.
Physical form
Affinity purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Ubiquitin-conjugating enzyme E2 A in 10 µg of HeLa cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | mouse, rat, human |
| species reactivity (predicted by homology) | primate (based on 100% sequence homology), sheep (based on 100% sequence homology), horse (based on 100% sequence homology), chicken (based on 100% sequence homology) |
| technique(s) | western blot: suitable |
| NCBI accession no. | NP_003327 |
| UniProt accession no. | P49459 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... UBE2A(7319) |
