General description
Millipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.
Millipore’s Chem-1 Parental Control Frozen Cells are designed for use as a negative control alongside Millipore’s GPCR-expressing Ready-to-Assay Frozen Cells in the Chem-1 background. Chem-1 cells lack endogenous expression of most GPCRs, although they endogenously express thrombin receptors that may be used to confirm responsiveness of the cells in calcium assays.
Biochem/physiol Actions
GPCR Cell Lines
Host cells: Chem-1
Protein Target: Not Applicable
Target Sub-Family: Control
Other Notes
Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
Fifty (50) mL of Media Component.
| biological source | rat |
| Quality Segment | 100 |
| manufacturer/tradename | Ready-to-Assay |
| technique(s) | calcium flux assay: suitable |
| detection method | fluorometric |
| shipped in | dry ice |
| storage temp. | -65 to -96°C |
