General description
VEGF-B, also known as Vascular endothelial growth factor B, VEGF-related factor, VRF, and encoded by the gene VEGFB/VRF, is an endothelial cell growth factor that has multiple splice variants and plays roles in angiogenesis but also in cardiac muscle function, coronary vascular development, chemotaxis of endothelial cells and mast cells, apoptosis regulation, platelet activation, cell division, wound healing and the activation of various signaling pathways. VEGFB is a secreted protein that stays associated with cells because of its ability to bind to heparin. VEGFB is expressed in all cells except for liver cells with the highest levels found in muscle and pancreatic cells. VEGFB levels in plasma are also being studied as a potential biomarker for ventricle damage after heart attacks. High levels of plasma VEGFB show little remodeling whereas patients with lower levels had significant ventricle remodeling, thus VEGFB may have clinical utility in the identification of patients at high risk of remodeling and therefore ventricle damage.
~40/32/25 kDa observed. The ~40kDa band observed is likely due to homo and/or hetero-dimers, the ~32kDa band observed is likely due to glycosylation, and the ~25kDa band is un-modified VEGF186 (Olofsson, B., et al. (1998). Proc Natl Acad Sci USA. 95(20):11709-11714).
Immunogen
Epitope: Unknown
KLH-conjugated linear peptide corresponding to human VEGF-B.
Application
Detect VEGF-B using this Anti-VEGF-B Antibody validated for use in western blotting & IHC (Paraffin).
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected VEGF-B in human skeletal muscle myocytes and vascular tissue and cardiac myocytes in human heart.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in human skeletal muscle tissue lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected VEGF-B in 10 µg of human skeletal muscle tissue lysate.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | human |
| concentration | 1 mg/mL |
| technique(s) | immunohistochemistry: suitable (paraffin),western blot: suitable |
| NCBI accession no. | NP_003368 |
| UniProt accession no. | P49765 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... VEGFB(7423) |
