General description
22 kDa calculated
OX40L (Tumor necrosis factor ligand superfamily member 4; or CD252) is the endogenous ligand of the OX40 receptor. OX40 is localized on activated T-cells and propagates the immune response by promoting division and survival of T-cells, thereby increasing the population of memory CD4 and CD8 T cells produced in response to antigen detection. The OX40/OX40L may also regulate cytokine production and suppress the activity of T-regulatory cells. OX40/OX40L is therefore an important mediator of the immune response. Previous studies have suggested that OX40 signaling maybe involved in the development of autoimmune diseases.
Immunogen
Mouse OX40L cDNA-transfected NRK cells
Application
Anti-OX40L (CD252) Antibody, clone RM134L detects level of OX40L (CD252) & has been published & validated for use in Flow Cytometry & IHC.
Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in mouse lung tissue. (Hoshino, A., et al. (2003). Eur. J. Immunol. 33:861–869.)
Biochem/physiol Actions
This antibody recognizes the extracellular domain of OX40L.
Physical form
Format: Purified
Analysis Note
Control
Mouse OX40L/L5178Y cells
Evaluated by Flow Cytometry in mouse OX40L/L5178Y cells.
Flow Cytometry Analysis: 10 µg of this antibody detected OX40L in mouse OX40L/L5178Y cells.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
| biological source | rat |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | RM134L, monoclonal |
| species reactivity | mouse |
| technique(s) | flow cytometry: suitable,immunohistochemistry: suitable |
| isotype | IgG2bκ |
| NCBI accession no. | NP_033478 |
| UniProt accession no. | P43488 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | mouse ... Tnfsf4(22164) |
