General description
57 kDa calculated
Nectin-1, also known as poliovirus receptor-related protein 1 (PRR1 or PVRL1) or CD111, is an immunoglobulin-like transmembrane cell adhesion protein that also seems to play a role in neural tube formation, with N-cadherin. Nectins are known for being the receptors for the herpes simplex virus. Nectin-1 is connected with the actin cytoskeleton through afadin inside the cell. It is predominantly found in human placenta although in mice, expression is wide-ranging. Nectin-1 seems to adhere primarily to cells expressing nectin-4 and vice versa.
Immunogen
Human CD111 transfected NIH/3T3 cells
Application
Immunofluorescence Analysis: A previous lot was used by an independent laboratory in IF (Cocchi, F., et al. (1998). Journal of Virology. 72(12):9992–10002).
This Anti-Nectin-1/PVRL1 (CD111) Antibody, clone R1.302 is validated for use in FC, IF for the detection of Nectin-1/PVRL1 (CD111).
Physical form
Format: Purified
Analysis Note
Control
Human TF-1 cells
Evaluated by Flow Cytometry in human TF-1 cells.
Flow Cytometry Analysis: 0.25 µg of this antibody detected Nectin-1/PVRL1 in human TF-1 cells.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | R1.302.12, monoclonal |
| species reactivity | human |
| technique(s) | flow cytometry: suitable,immunofluorescence: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NP_002846 |
| UniProt accession no. | Q15223 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... PVRL1(5818) |
