General description
47kDa
Immunogen
Purified GST fusion protein produced in E. coli., corresponding to human Nck amino acid sequence 1 to 254
Application
Detect Nck using this Anti-Nck Antibody validated for use in IP & WB.
Research Category
Signaling
Research Sub Category
Cytoskeletal Signaling
Biochem/physiol Actions
Nck
Physical form
0.1M Tris-glycine, pH 7.4, 0.15M NaCl containing 0.05% sodium azide
Format: Purified
Protein G Chromatography
Preparation Note
2 years at -20°C
Analysis Note
Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
routinely evaluated by immunoblot on human A431 carcinoma cells, mouse 3T3/A31 fibroblasts or rat L6 skeletal fibroblasts
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | mouse, rat, human |
| manufacturer/tradename | Upstate® |
| technique(s) | immunoprecipitation (IP): suitable,western blot: suitable |
| isotype | IgG |
| NCBI accession no. | NM_010878 |
| UniProt accession no. | P16333 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... NCK1(4690) |
