General description
Purified mouse monoclonal antibody. Recognizes the ~73 kDa p73 protein lacking the N-terminal transactivation domain, ΔpNp73.
Recognizes the ~73 kDa ΔNp73 protein.
This Anti-ΔNp73 Mouse mAb (38C674) is validated for use in Immunoblotting for the detection of ΔNp73.
Immunogen
Human
a synthetic peptide (LYVGDPARHLAT) corresponding to amino acids 2-13 of human ΔNp73
Application
Immunoblotting (1 g/ml, chemiluminescence)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In PBS
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
Cell lines transfected with ΔNp73
Other Notes
Does not cross-react with full-length p73. Antibody should be titrated for optimal results in individual systems.
Levrero, M., et al. 2000 J. Cell Sci.113, 1661.
Yang, A., et al. 2000. Nature404, 99.
Ueda, Y., et al. 1999. Oncogene18, 4993.
Kaghad, M., et al. 1997. Cell90, 809.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
| biological source | mouse |
| Quality Segment | 100 |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 38C674, monoclonal |
| form | liquid |
| contains | ≤0.1% sodium azide as preservative |
| species reactivity | human |
| manufacturer/tradename | Calbiochem® |
| storage condition | OK to freeze,avoid repeated freeze/thaw cycles |
| dilution | (Immunoblotting (1 µg/mL, chemiluminescence)) |
| isotype | IgG1 |
| shipped in | wet ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| Gene Information | human ... TP53(7157) |
