General description
PRKC apoptosis WT1 regulator protein, also known as Prostate apoptosis response 4 protein (Par-4), and encoded by the gene name PAWR or PAR4, is a leucine zipper and death domain containing protein whose levels increase in neurons undergoing apoptosis as a result of trophic factor withdrawal or exposure to oxidative and metabolic insults. PAWR levels are reported to be increased in their lumbar spinal cord specimens further suggesting a role in neuronal degeneration. PAWR is localized to the cytoplasm in the absence of apoptosis signal and in normal cells and localized to the nucleus in most cancer cells. The tumor suppressor WT1 represses and activates transcription. The loss and/or imbalance of the dual transcriptional activity of WT1 may contribute to Wilms tumor. PAWR is a WT1 interacting protein that also functions as a transcriptional repressor.
~43 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Immunogen
Purified recombinant fragment of human PAWR expressed in E. Coli
Application
Detect PAWR using this Anti-PAWR, clone 3G9H7 Antibody validated for use in western blotting & IHC.
Immunohistochemistry Analysis: A 1:200-1:1,000 dilution from a representative lot detected PAWR in human brain and breast carcinoma tissues.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Physical form
Mouse monoclonal IgG1 ascitic fluid with up to 0.1% sodium azide.
Unpurified
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Evaluated by Western Blotting in Full-length Trx-PAWR recombinant protein and HeLa cell lysate.
Western Blotting Analysis: A 1:500-1:2,000 dilution of this antibody detected PAWR in Full-length Trx-PAWR recombinant protein and HeLa cell lysate.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| clone | 3G9H7, monoclonal |
| species reactivity | human |
| technique(s) | immunohistochemistry: suitable,western blot: suitable |
| isotype | IgG1 |
| UniProt accession no. | Q96IZ0 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... PAWR(5074) |
