General description
Homeobox protein Hox-C10 (UniProt Q9NYD6; also known as Homeobox protein Hox-3I) is encoded by the HOXC10 (also known as HOX3I) gene (Gene ID 3226) in human. HOXC10 belongs to a family of highly conserved homeobox transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, with HOXC genes located in a cluster on chromosome 12. Hox-C10 is also implicated to be involved in replication, where it binds in a structure-dependent manner to origins of replication and contributes to the assembly of replicative complexes. HOXC10 transcription is reported to be silenced in two estrogen deprivation-resistent cell lines, C4-12 and LTED, derived from MCF-7 breast cancer cells as a result of epigenetic hypermethylation as well as additional repressive histone modifications. Evidence indicates that Hox-C10 expression is repressed by estrogen in vitro and in vivo to promote tumor growth. Short-term estrogen withdrawal restores Hox-OXC10 expression to induce growth arrest and apoptosis, but its long-term absence results in permanent repression as a result of epigenetic methylation and the development of estrogen-independent cancer cells.
~38 kDa observed. 38.07 kDa calculated. Uncharacterized band(s) may appear in some lysates.
Immunogen
Epitope: Internal.
GST-tagged recombinant human Hox-C10 internal fragment.
Application
Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected Hox-C10 in A431, HeLa, HepG2, and NIH/3T3 cells.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Hox-C10 in human colon cancer tissue, as well as in normal kidney and panreas tissue sections.
Research Category
Apoptosis & Cancer
Research Sub Category
Transcription Factors
This Anti-Hox-C10 Antibody, clone 2F11.1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin), Immunocytochemistry for the detection of Hox-C10.
Biochem/physiol Actions
Clone 2F11.1 recognizes an internal epitope of human homeobox protein Hox-C10, also known as Hox-3I.
Physical form
Format: Purified
Protein G purified.
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in MCF-7 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Hox-C10 in 10 µg of MCF-7 cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 2F11.1, monoclonal |
| species reactivity | mouse, human |
| technique(s) | immunocytochemistry: suitable,immunohistochemistry: suitable (paraffin),western blot: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NP_059105 |
| UniProt accession no. | Q9NYD6 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... HOXC10(3226) |
