General description
Fas-associated protein 1 (FAF1) is a member of the TNF receptor superfamily that plays a critical role in apoptosis during development and immune function. FAF1 has two amino-terminal domains with structural homology to ubiquitin. FAF1 has been observed to be one of the components of the death-inducing signaling complex (DISC) during Fas-mediated apoptosis and can bind to caspase-8 and FADD. Additionally, FAF1 has been shown to suppress the activation of the NF-kappaB transcription factor. FAF1 is also known to interact with the inflammatory signaling PYRIN-containing Apaf-1-like proteins (PYPAFs, also called NALPs) such as PYPAF1, PYPAF2 (NALP2), and PYPAF7, suggesting FAF1 may also be involved in the inflammation pathway. FAF1 is widely expressed with highest expression observed in testis, slightly less abundant in skeletal muscle and heart, followed by prostate, thymus, ovary, small intestine, and colon.
~78 kDa observed. Uncharacterized bands may appear in some lysate(s). Uniprot describes 2 isoforms at ~57 kDa and ~74 kDa
Immunogen
Epitope: C-terminus
KLH-conjugated linear peptide corresponding to the C-terminus of human FAF1.
Application
Anti-FAF1 Antibody is a highly specific rabbit polyclonal antibody, that targets Fas-associated protein 1 & has been tested in western blotting & IHC.
Immunohistochemistry Analysis: 2.5 µg/mL from a representative lot detected FAF1 in rat spleen tissue.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Biochem/physiol Actions
This antibody recognizes the C-terminus of FAF1.
Physical form
Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Jurkat cell lysate
Evaluated by Western Blotting in Jurkat cell lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected FAF1 in 15 µg of Jurkat cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | rat, human |
| technique(s) | immunohistochemistry: suitable,western blot: suitable |
| UniProt accession no. | Q9UNN5 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... FAF1(11124) |
