General description
A cell-permeable DM-PIT-1 (Cat. No. 524619) analog that is more selective and potent than DM-PIT-1 against PH domain-mediated PIP3 (Cat. No. 524615) binding to Akt1 (IC50 = 13.4 and 27.1 µM, respectively) and PDK1 (IC50 = 52.3 and 80.5 µM, respectively), while being much less active than DM-PIT-1 against PIP3 GRP1 PH domain binding (12% vs 84% inhibition with 100 µM respective compound). PIT-7 is also shown to be more effective than DM-PIT-1 in inhibiting human glioblastoma U87MG survival (IC50 = 6.6 vs. 33.9 µM, respectively) and cellular Akt Ser473 phosphorylation in cultures in vitro.
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Other Notes
Miao, B., et al. 2010. Proc. Natl. Acad. Sci. USA107, 20126.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
| Quality Segment | 100 |
| assay | ≥95% (HPLC) |
| form | semisolid |
| manufacturer/tradename | Calbiochem® |
| storage condition | OK to freeze,protect from light |
| color | yellow-white |
| solubility | DMSO: 100 mg/mL |
| shipped in | ambient |
| storage temp. | 2-8°C |
