Immunogen
A 17 amino acid peptide corresponding to amino acids 175-191 from soluble cytoplasmic human PGP9.5.
Application
Anti-Protein Gene Product 9.5 Antibody detects level of Protein Gene Product 9.5 & has been published & validated for use in IH.
Immunohistochemistry: 1:500.
Optimal working dilutions must be determined by end user.
blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 4°C for 18-24 hours with AB5898 (1:500). After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.
Research Category
Neuroscience
Research Sub Category
Neuronal & Glial Markers
Biochem/physiol Actions
Recognizes Protein Gene Product 9.5 (PGP9.5).
Physical form
Liquid. Contains 0.05% sodium azide.
Unpurified
Preparation Note
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Brain tissue
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | guinea pig |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | serum |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | human, rat, mouse |
| manufacturer/tradename | Chemicon® |
| technique(s) | immunohistochemistry: suitable |
| NCBI accession no. | NM_004181.3 |
| UniProt accession no. | P09936 |
| shipped in | dry ice |
| target post-translational modification | unmodified |
| Gene Information | human ... UCHL1(7345) mouse ... Uchl1(22223) rat ... Uchl1(29545) |
