General description
21 kDa
RhoG (ARGH) is a member of the Ras super-family of small G-proteins and a member of the Rac subfamily of Rho Family of GTPases. RhoG is a small G-protein (GTPase) who is activated by a GEF (guanine nucleotide exchange factor). The GEF displaces GDP from the binding site of RhoG and allows GTP to bind in it place rendering the protein active. This active RhoG binds downstream effector molecules that include ELMO and DOCK180. RhoG then cleaves GTP to GDP making it inactive. RhoG is believed to be crucial for integrin-mediated Rac activation and cell spreading. RhoG is believed to be active upstream of Rac and work independently of Rac.
Immunogen
KLH synthetic peptide encompassing amino acids CQQDGVKEVFAEAVRAVLNPT (RhoG 160-180)
Application
Anti-RhoG Antibody, clone 1F3 B3 E5 is an antibody against RhoG for use in WB.
Biochem/physiol Actions
Recognizes RhoG
Physical form
Format: Purified
Purified mouse monoclonal IgG2b in storage buffer containing PBS with 0.05% sodium azide.
Analysis Note
Routinely evaluated by western blot on NIH/3T3 cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
| biological source | mouse |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | 1F3 B3 E5, monoclonal |
| species reactivity | mouse, rat, human |
| manufacturer/tradename | Upstate® |
| technique(s) | western blot: suitable |
| isotype | IgG2bκ |
| NCBI accession no. | NM_001665 |
| UniProt accession no. | P84095 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... RHOG(391) |
