General description
Beta-Pix (ARHGEF7, βPix, COOL) is a Rho guanine nucleotide exchange factor (GEF). Beta-Pix is a p21-activated protein kinase (PAK)-interacting exchange factor (Pix, COOL, p85SPR) that has been identified as a putative guanine nucleotide exchange factor (GEF) for Rac/Cdc42. The variants of Beta-Pix isoforms exhibit different tissue distributions. Beta-Pix-a is expressed ubiquitously in all the tissues examined, and other isoforms are expressed mainly in the central nervous system. PAK, a Ser/Thr kinase, is an important mediator of Rho family activity interacts with Beta-Pix at the SH3 domain of Beta-Pix though its proline rich region. This interaction is thought to affect the GTP hydrolysis rate of Rac and Cdc42.
~80 kDa observed
Immunogen
Epitope: SH3 domain
GST-tagged recombinant protein corresponding to the SH3 domain of human Beta-Pix, SH3 Domain.
Application
Detect Beta Pix using this Anti-Beta Pix antibody, SH3 Domain validated for use in western blotting.
Research Category
Signaling
Research Sub Category
Signaling Neuroscience
Western Blotting Analysis: 1 µg/mL from a representative lot detected Beta-Pix, SH3 Domain in 10 µg of PC-12 cell lysate.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in Jurkat cell lysate.
Western Blotting Analysis: 0.2 µg/mL of this antibody detected Beta-Pix, SH3 Domain in 10 µg of Jurkat cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 07-1450
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Level | 100 |
| conjugate | unconjugated |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| purified by | affinity chromatography |
| species reactivity | mouse, rat, human |
| technique(s) | western blot: suitable |
| NCBI accession no. | NP_001106983 |
| UniProt accession no. | Q14155 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... ARHGEF7(8874) |
