General description
Research area: Apoptosis
Protein Target: PKCα
Target Sub-Family: AGC
PKCα
Recombinant, full-length human PKCα, containing N-terminal His6 tag
Application
Active, recombinant, full-length human PKCα, containing N-terminal His6 tag. For use in Kinase Assays.
Biochem/physiol Actions
Protein kinase C-α (PKCα), an isoform of protein kinase C, exhibits an anti-apoptotic and pro-proliferative function. It is commonly upregulated in tumor cells, and its depletion has been associated with apoptosis induction. The anti-apoptotic effects of PKCα are mediated through mechanisms such as phosphorylation of the anti-apoptotic protein B-cell lymphoma 2 (Bcl-2) and activation of the pro-survival protein kinase AKT. The inhibition of PKCα leads to the activation of a PKCδ-dependent apoptotic pathway in salivary epithelial cells. PKCα regulates cell cycle progression in glioma cells by increasing the expression of p21 cyclin-dependent kinase inhibitor 1 (p21 (Waf1/Cip1)), a protein involved in cell cycle regulation.
Packaging
Also available in 10 μg size and in bulk
Also available in 250 µg size --call for pricing and availability and reference catalog number 14-484M when ordering the 250 µg size.
Physical form
Ni2+/NTA-agarose
Preparation Note
1 year at -70°C
Analysis Note
routinely evaluated by phosphorylation of Histone H1.
Other Notes
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | human |
| Quality Segment | 100 |
| recombinant | expressed in baculovirus infected Sf21 cells |
| product line | Upstate® |
| specific activity | (For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.) |
| shelf life | 1 yr |
| mol wt | Mw 78 kDa |
| purified by | (Ni2+/NTA-agarose) |
| species reactivity | human |
| manufacturer/tradename | Upstate® |
| technique(s) | activity assay: suitable (kinase) |
| accession no. | (FUNCTION: SwissProt: P17252 # PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. ),NM_002737.2 |
| NCBI accession no. | NM_002737.2 |
| UniProt accession no. | P17252 |
| storage temp. | −70°C |
| Gene Information | human ... PRKCA(5578) |
