General description
GABA(A) receptor subunit gamma-2 is a protein belonging to a subclass of GABA receptors known as GABA (A). GABA (A) receptors are pentameric chloride channels composed of subunits belonging to different homology classes, i.e. α, β, γ, δ, θ, ε, π, and act as receptors for GABA, a crucial inhibitory neurotransmitter localized within the brain. GABA (A) gamma 2 subunit consists of 5 cysteine residues and carries the binding site for benzodiazepine. The subunit functions to promote receptor maintenance and clustering at the synapse, interacts with GABA receptor associated proteins such as GODZ and GABAWRAP, affects GABA (A) receptor conductance and kinetics, and is a contributing component to receptors participating in both phasic and tonic inhibition. Several mutations in the GABA(A) receptor subunit gamma-2 expression have been linked to epileptic syndromes that result in fibrile seizures.
~60 kDa observed
Immunogen
GST-tagged recombinant protein corresponding to rat GABA(A) receptor subunit gamma-2.
Application
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
This Anti-GABA(A) receptor subunit gamma-2 Antibody is validated for use in WB for the detection of GABA(A) receptor subunit gamma-2.
Biochem/physiol Actions
This antibody recognizes GABA(A) receptor subunit gamma-2.
Physical form
Rabbit polyclonal serum containing 0.05% sodium azide.
Unpurified
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
PC12 cell lysate
Evaluated by Western Blot in PC12 cell lysate.
Western Blot Analysis: 1:1,000 dilution of this antibody detected GABA(A) receptor subunit gamma-2 on 10 µg of PC12 cell lysate.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | rabbit |
| Quality Segment | 100 |
| conjugate | unconjugated |
| antibody form | serum |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | rat, human |
| technique(s) | western blot: suitable |
| NCBI accession no. | NP_899156 |
| UniProt accession no. | P18508 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... GABRG2(2566) |
