General description
An internally quenched fluorogenic peptide substrate that contains the α-secretase cleavage site of β-amyloid precursor protein (APP). Shown to be an effective substrate for yapsin-2, a glycosyl-phosphatidylinositol-linked aspartyl protease found in yeast (Vmax/Km = 0.031 min-1). The cleavage has been shown to occur at the Lys-Leu bond. Excitation max: ~340 nm; emission max: ~490 nm.
Biochem/physiol Actions
Cell permeable: no
Primary Target
Substrate containing the α-secretase cleavage site of β-Amyloid Precursor Protein (APP)
Product does not compete with ATP.
Reversible: no
Packaging
Packaged under inert gas
Other Notes
Ac-Arg-Glu(EDANS)-Val-His-His-Gln-Lys-Leu-Val-Phe-Lys(DABCYL)-Arg-OH
Komano, H., et al. 1999. J. Biol. Chem. 274, 24431.
Komano, H., et al. 1998. J. Biol. Chem. 273, 31648.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
| Quality Level | 100 |
| assay | ≥95% (HPLC) |
| form | solid |
| manufacturer/tradename | Calbiochem® |
| storage condition | OK to freeze,desiccated (hygroscopic),protect from light |
| color | red |
| solubility | DMSO: 5 mg/mL |
| fluorescence | λex ~340 nm,λem ~490 nm |
| shipped in | ambient |
| storage temp. | −20°C |
