General description
N-terminal GST-tagged, recombinant human NFkB, p50 subunit (D434-969), expressed in E. coli. Purified using glutathione agarose chromatography. MW = 74kDa.
Application
DNA Binding Assay
Physical form
50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.1mM EGTA, 0.1% βmercaptoethanol, 0.03% Brij-35, 0.2mM PMSF, 1mM benzamidine and 50% glycerol
Preparation Note
1 year at -20°C
Analysis Note
routinely evaluated in a DNA binding assay
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | human |
| Quality Level | 100 |
| recombinant | expressed in E. coli |
| form | liquid |
| manufacturer/tradename | Upstate® |
| NCBI accession no. | NM_003998.2 |
| UniProt accession no. | P19838 |
| shipped in | wet ice |
| Gene Information | human ... NFKB1(4790) |
