General description
~17 kDa observed.
Allograft inflammatory factor 1 (UniProt P55008; also known as AIF-1, Daintain, Interferon gamma responsive transcript, Ionized calcium-binding adapter molecule 1, Protein G1) is encoded by the AIF1 (also known as G1, IBA1) gene (Gene ID 199) in human. Iba1/AIF1 is a 17 kDa interferon-gamma- (IFN-gamma-) inducible, Ca(2+)-binding EF-hand protein. Iba1/AIF1 expression is mostly limited to the macrophage/monocyte lineage, and is augmented by cytokines, such as IFN-gamma. It is reported to be involved in neuronal function, inflammatory responses, allograft rejection, autoimmunity, and macrophage activation/function. It is also shown to be expressed in microglial cells in autominnume disesase models such as experimental autoimmune encephalomyelitis, neuritis and uveitis.
Application
Anti-Iba1/AIF1 Antibody, clone 20A12.1, Alexa Fluor™ 647 Conjugate is an antibody against AIF1 for use in Immunofluorescence.
The unconjugated antibody (Cat. No. MABN92) is shown to be suitable also for Western blotting and immunohistochemistry applications.
Analysis Note
Evaluated by Immunofluorescence in human spleen tissue.
Immunofluorescence Analysis: A 1:50 dilution of this antibody detected Iba1/AIF1 in human spleen tissue.
Other Notes
Concentration: Please refer to lot specific datasheet.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
| biological source | mouse |
| Quality Level | 100 |
| conjugate | ALEXA FLUOR™ 647 |
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | 20A12.1, monoclonal |
| species reactivity | mouse, human, rat |
| technique(s) | immunofluorescence: suitable |
| isotype | IgG1κ |
| NCBI accession no. | NP_001614 |
| UniProt accession no. | P55008 |
| shipped in | wet ice |
| target post-translational modification | unmodified |
| Gene Information | human ... AIF1(199) |
