General description
Alpha-synuclein (UniProt: P37840; also known as Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, NACP) is encoded by the SNCA (also known as NACP, PARK1) gene (Gene ID: 6622) in human. Alpha-synuclein is a membrane-bound protein in dopaminergic neurons that is involved in the regulation of dopamine release and transport. It is also reported to induce fibrillization of microtubule-associated protein tau and reduces neuronal responsiveness to various apoptotic stimuli. Aggregation of alpha-synuclein is known to be a causative factor in the development of Parkinson′s disease (PD), which leads to the degeneration of dopaminergic neurons in the substantia nigra (SN) in the midbrain region. Aggregates of alpha-synuclein are present in Lewy bodies. Alpha-synuclein can be phosphorylated, predominantly on serine residues by casein kinase 1. Phosphorylation of Ser129 is reported to be selective and extensive in synucleinopathy lesions and promotes insoluble fibril formation. Mutations in the SNCA gene produce defective alpha-synuclein that induces conformational changes making it more prone to self-aggregation and deposition in Lewy bodies. Mutant forms of alpha-synuclein impair synaptic vesicle formation, elevate cytoplasmic levels of dopamine, and increases superoxide radicals, which lead to oxidative stress and misfolding of alpha-synuclein.
~75 kDa observed; 14.46 kDa calculated (monomers). Uncharacterized bands may be observed in some lysate(s).
Immunogen
Synuclein oligomers from full length human wild-type alpha-synuclein.
Application
Anti-alpha-Synuclein, oligomer-specific Syn33 Antibody, Cat. No. ABN2265, is a highly specific rabbit polyclonal antibody that targets aggregted alpha-synuclein and has been tested in ELISA, Inhibition, Immunofluorescence, and Western Blotting.
Immunofluorescence Analysis: A representative lot detected alpha-Synuclein, oligomers in brain cortices from Parkinson s disease (PD) and dementia with Lewy bodies (DLB) patients (Sengupta, U., et. al. (2015). Biol Psychiatry. 78(10):672-83).
Western Blotting Analysis: A representative lot detected recombinant alpha-Synuclein, oligomers. (Sengupta, U., et. al. (2015). Biol Psychiatry. 78(10):672-83).
Western Blotting Analysis: A representative lot detected recombinant alpha-Synuclein, oligomer. (Courtesy of Dr. Rakez Kayed′s laboratory at University of Texas Medical Branch, Galveston).
ELISA Analysis: A representative lot detected alpha-Synuclein, oligomers in ELISA application (Sengupta, U., et. al. (2015). Biol Psychiatry. 78(10):672-83).
Inhibition Analysis: A representative lot inhibited cytotoxicity exerted alpha-synuclein oligomers in human neuroblastoma SH-SY5Y cells measured by MTT-based assay (Sengupta, U., et. al. (2015). Biol Psychiatry. 78(10):672-83).
Biochem/physiol Actions
This rabbit polyclonal antibody specifically recognizes aggregated form of alpha-synuclein.
Physical form
Format: Purified
Purified rabbit polyclonal antibody in PBS.
Analysis Note
Evaluated by Western Blotting in Alpha Synuclein aggregate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected alpha-Synuclein in alpha Synuclein aggregates.
Other Notes
Concentration: Please refer to lot specific datasheet.
| biological source | rabbit |
| Quality Level | 100 |
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| species reactivity | human |
| technique(s) | ELISA: suitable,immunofluorescence: suitable,inhibition assay: suitable,western blot: suitable |
| isotype | IgG |
| NCBI accession no. | NP_000336 |
| UniProt accession no. | P37840 |
| shipped in | ambient |
| target post-translational modification | unmodified |
| Gene Information | human ... SNCA(6622) |
