General description
CD40 Ligand (CD40L/TRAP) is a type II membrane protein which, effectuates the helper function of T cells on resting B cells. GF101 is a 16.3 kDa protein containing 149 amino acid residues comprising the receptor binding TNF-like domain of CD40L.
Product Source: Recombinant human
Physical form
Sterile filtered and lyophilized from 10 mM Sodium Phosphate, pH 7.5.
Preparation Note
The lyophilized protein is stable for a few weeks for at room temperature but is best stored at -20ºC. Reconstitute in water to 0.1-1.0 mg/ml. This solution can be diluted into other aqueous buffers and stored at 2-8ºC for one week or -20ºC for future use. Reconstituted sCD40L/TRAP should be stored at -20ºC in undiluted aliquots for up to 6 months. Freeze/thaw cycles will result in significant loss of activity.
Analysis Note
Specific Activity: GF101 is fully biologically active as determined by its ability to stimulate the proliferation of HEK-BlueTMCD40 reporter cells.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
| biological source | human |
| Quality Segment | 100 |
| assay | >98% (SDS-PAGE and HPLC) |
| manufacturer/tradename | Chemicon® |
| technique(s) | cell culture | mammalian: suitable |
| impurities | <0.1 ng/μg Endotoxin (of sCD40L/TRAP; 1EU/μg) |
| NCBI accession no. | NM_000074.2 |
| UniProt accession no. | P29965 |
| shipped in | dry ice |
| Gene Information | human ... CD40LG(959) |
